HMW Kininogen Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31340

Novus Biologicals, part of Bio-Techne
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NBP1-31340
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HMW Kininogen Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMW Kininogen. The exact sequence is proprietary.

Localization

Secreted, extracellular space

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMW Kininogen Antibody

Western Blot: HMW Kininogen Antibody [NBP1-31340]

Western Blot: HMW Kininogen Antibody [NBP1-31340]

Western Blot: HMW Kininogen Antibody [NBP1-31340] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HMW Kininogen Antibody [NBP1-31340]

Immunocytochemistry/ Immunofluorescence: HMW Kininogen Antibody [NBP1-31340]

Immunocytochemistry/Immunofluorescence: HMW Kininogen Antibody [NBP1-31340] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HMW Kininogen Antibody [NBP1-31340]

Immunohistochemistry-Paraffin: HMW Kininogen Antibody [NBP1-31340]

Immunohistochemistry-Paraffin: HMW Kininogen Antibody [NBP1-31340] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Applications for HMW Kininogen Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HMW Kininogen

High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein (see MIM 147910)-kinin system. The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing. Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3. However, HMWK contains a 56-kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4-kD light chain that consists of domain 5L. In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide. BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin (see MIM 601699) from endothelial cells. During vascular damage, BK stimulates smooth muscle proliferation and intimal hypertrophy. Release of BK from HMWK generates a 2-chain HMWK, termed HMWKa, containing the heavy and light chains joined by a disulfide bond (Merkulov et al., 2008 [PubMed 18000168]).[supplied by OMIM]

Alternate Names

Alpha-2-thiol proteinase inhibitor, BDK, bradykinin, Fitzgerald factor, High molecular weight kininogen, kininogen, kininogen 1, kininogen-1, KNGHMWK, Williams-Fitzgerald-Flaujeac factor

Gene Symbol

KNG1

Additional HMW Kininogen Products

Product Documents for HMW Kininogen Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMW Kininogen Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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