HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45270

Novus Biologicals, part of Bio-Techne
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NBP2-45270
NBP2-45270SS
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BSA Free
NBP2-45269
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (96%). Backed by our 100% Guarantee.

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3B11NB

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all HNF-3 alpha/FoxA1 Antibodies »

Product Summary for HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free

Immunogen

Partial recombinant human FOXA1 protein (between amino acids 350-500) .

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free

Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB)Azide and BSA Free [NBP2-45270]

Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB)Azide and BSA Free [NBP2-45270]

Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide Free [NBP2-45270] - Whole cell protein from PC3 (1) and MCF7 (2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4 ug/ml anti-hFOXA1 (3B11NB) in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image using the standard format of this product.
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free [NBP2-45270]

Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free [NBP2-45270]

Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide Free [NBP2-45270] - FOXA1 (3B11NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively Image using the standard format of this product.
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free [NBP2-45270]

Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free [NBP2-45270]

Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide Free [NBP2-45270] - Analysis of FOXA1 (3B11NB) in human prostate. Image using the standard format of this product.
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Applications for HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

25 ug/ml

Immunocytochemistry/ Immunofluorescence

1:25 - 1:50

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2 - 4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-3 alpha/FoxA1

FoxA1 (forkhead box A1), also called HNF-3a (hepatocyte nuclear factor 3 alpha) or TCF3A (transcription factor 3A) is a 473 amino acid (aa), 49 kDa (predicted) protein of the forkhead nuclear transcription factor family. It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors. FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines. Within the sequence used as an immunogen, human FoxA1 shares 93% and 86% aa identity with mouse and rat FoxA1, respectively.

Long Name

Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1

Alternate Names

FoxA1, HNF3 alpha, TCF3A

Gene Symbol

FOXA1

Additional HNF-3 alpha/FoxA1 Products

Product Documents for HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (3B11NB) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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