HNF-3 alpha/FoxA1 Antibody (3B2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26572
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3B2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HNF-3 alpha/FoxA1 Antibody (3B2)
Immunogen
A synthesized peptide derived from Human HNF-3 alpha/FoxA1 [UniProt P55317]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (3B2)
Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] -
Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Immunoprecipitating HNF-3 alpha/FoxA1 in Hela whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26572 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26572 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] -
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Image of HNF-3 alpha/FoxA1 Antibody (3B2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] -
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Image of HNF-3 alpha/FoxA1 Antibody (3B2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Applications for HNF-3 alpha/FoxA1 Antibody (3B2)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: HNF-3 alpha/FoxA1
Long Name
Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1
Alternate Names
FoxA1, HNF3 alpha, TCF3A
Gene Symbol
FOXA1
Additional HNF-3 alpha/FoxA1 Products
Product Documents for HNF-3 alpha/FoxA1 Antibody (3B2)
Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (3B2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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