HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54575

Novus Biologicals, part of Bio-Techne
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NBP2-54575-100ug
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Key Product Details

Species Reactivity

Validated:

Human, Rat (Negative)

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # FOXA1/1514

Format

Azide and BSA Free

Concentration

1 mg/ml

View all HNF-3 alpha/FoxA1 Antibodies »

Product Summary for HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free

Immunogen

Recombinant human HNF-3 alpha/FoxA1 protein fragment (aa372-472) (exact sequence is proprietary) (Uniprot: P55317 )

Reactivity Notes

Does not react with Rat. Others not known.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53338).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free [NBP2-54575]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free [NBP2-54575]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) [NBP2-54575] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1514).
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free [NBP2-54575]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free [NBP2-54575]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) [NBP2-54575] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1514).

Applications for HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5 - 1 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml

Western Blot

0.5 - 1 ug/ml
Application Notes
Immunohistology (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: HNF-3 alpha/FoxA1

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1

Alternate Names

FoxA1, HNF3 alpha, TCF3A

Gene Symbol

FOXA1

Additional HNF-3 alpha/FoxA1 Products

Product Documents for HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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