HNF-3 alpha/FoxA1 Antibody (FOXA1/1519)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53331

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # FOXA1/1519

Concentration

0.2 mg/ml

View all HNF-3 alpha/FoxA1 Antibodies »

Product Summary for HNF-3 alpha/FoxA1 Antibody (FOXA1/1519)

Immunogen

Recombinant fragment of human HNF-3 alpha/FoxA1 protein (aa372-472) (exact sequence is proprietary) (Uniprot: P55317 )

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54413)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (FOXA1/1519)

Western Blot: HNF-3 alpha/FoxA1 Antibody (FOXA1/1519) [NBP2-53331]

Western Blot: HNF-3 alpha/FoxA1 Antibody (FOXA1/1519) [NBP2-53331]

Western Blot: HNF-3 alpha/FoxA1 Antibody (FOXA1/1519) [NBP2-53331] - Western Blot Analysis of human HeLa cell lysate using HNF-3 alpha/FoxA1 Antibody (FOXA1/1519).
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (FOXA1/1519) [NBP2-53331]

Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (FOXA1/1519) [NBP2-53331]

Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (FOXA1/1519) [NBP2-53331] - Immunofluorescent analysis of PFA-fixed MCF-7 cells labeled with HNF-3 alpha/FoxA1 Mouse Monoclonal Antibody (HNF-3 alpha/FoxA1/1519) followed by goat anti-mouse IgG-CF488. Counterstain: phalloidin.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1519) [NBP2-53331]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1519) [NBP2-53331]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1519) [NBP2-53331] - Formalin-fixed, paraffin-embedded Rat Colon stained with FOXA1 Monoclonal Antibody (FOXA1/1519).
View 2 more images

Applications for HNF-3 alpha/FoxA1 Antibody (FOXA1/1519)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HNF-3 alpha/FoxA1

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1

Alternate Names

FoxA1, HNF3 alpha, TCF3A

Gene Symbol

FOXA1

UniProt

P55317

Additional HNF-3 alpha/FoxA1 Products

Product Documents for HNF-3 alpha/FoxA1 Antibody (FOXA1/1519)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (FOXA1/1519)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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