HNF-3 alpha/FoxA1 Antibody (JF10-02), Novus Biologicals
Bio-Techne includes Novus Biologicals | Catalog # NBP2-66889
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JF10-02
Concentration
1 mg/ml
Product Summary for HNF-3 alpha/FoxA1 Antibody (JF10-02)
Immunogen
Recombinant protein within Human HNF-3 alpha/FoxA1 aa 320-472 / 472. (SwissProt: P55317 Human; SwissProt: P35582 Mouse; SwissProt: P23512 Rat)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (JF10-02)
Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Staining FOXA1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western Blot: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -
Western Blot: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Analysis of FOXA1 on different lysates with Rabbit anti-FOXA1 antibody at 1/2,000 dilution.Lane 1: MCF7 cell lysateLane 2: HepG2 cell lysateLane 3: A549 cell lysateLane 4: LNCaP cell lysateLysates/proteins at 20 ug/Lane.Predicted band size: 49 kDaObserved band size: 49 kDaExposure time: 5 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -
Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Analysis of LNCaP cells labeling FOXA1 with Rabbit anti-FOXA1 antibody at 1/250 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FOXA1 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
Applications for HNF-3 alpha/FoxA1 Antibody (JF10-02)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
IF-Cell-1:50-1:250, IF-Tissue-1:50-1:1,000
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:2,000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HNF-3 alpha/FoxA1
Long Name
Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1
Alternate Names
FoxA1, HNF3 alpha, TCF3A
Gene Symbol
FOXA1
Additional HNF-3 alpha/FoxA1 Products
Product Documents for HNF-3 alpha/FoxA1 Antibody (JF10-02)
Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (JF10-02)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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