HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07177
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177]](https://resources.bio-techne.com/images/products/HNF-3-alpha-FoxA1-Antibody-rFOXA1-1515-Immunohistochemistry-Paraffin-NBP3-07177-img0002.jpg "Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Flow Cytometry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rFOXA1/1515
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human HNF-3 alpha/FoxA1 protein fragment (around aa372-472) (exact sequence is proprietary) (Uniprot: P55317)
Localization
Nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08920)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515)
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177]
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HNF-3 alpha/FoxA1 Mouse Recombinant Monoclonal Antibody (rHNF-3 alpha/FoxA1/1515).![Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177]](https://resources.bio-techne.com/images/products/HNF-3-alpha-FoxA1-Antibody-rFOXA1-1515-Flow-Cytometry-NBP3-07177-img0001.jpg "Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177]")
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177]
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177] - Flow Cytometric Analysis of PFA-fixed permeabilized MCF-7 cells using HNF-3 alpha/FoxA1 Mouse Recombinant Monoclonal Antibody (rHNF-3 alpha/FoxA1/1515). followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: HNF-3 alpha/FoxA1
Long Name
Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1
Alternate Names
FoxA1, HNF3 alpha, TCF3A
Gene Symbol
FOXA1
UniProt
Additional HNF-3 alpha/FoxA1 Products
Product Documents for HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515)
Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...