HNF-3 alpha/FoxA1 Antibody (S04-5A7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19637

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19637-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-5A7

Concentration

0.3 mg/ml

View all HNF-3 alpha/FoxA1 Antibodies »

Product Summary for HNF-3 alpha/FoxA1 Antibody (S04-5A7)

Immunogen

Recombinant protein of human HNF-3 alpha/FoxA1 (Uniprot # P55317)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HNF-3 alpha/FoxA1 Antibody (S04-5A7)

Western Blot: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637]

Western Blot: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637]

Western Blot: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637] - Western blot detection of HNF-3 alpha/FoxA1 in C6 cell lysates using NBP3-19637 (1:1000 diluted). Predicted band size: 49kDa. Observed band size: 49kDa.
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637]

Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637]

Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637] - Immunofluorescence of HNF-3 alpha/FoxA1 (green) in MCF-7 using NBP3-19637 at dilution 1/20, and DAPI (blue)

Applications for HNF-3 alpha/FoxA1 Antibody (S04-5A7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20

Western Blot

1/1000-1/5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-3 alpha/FoxA1

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1

Alternate Names

FoxA1, HNF3 alpha, TCF3A

Gene Symbol

FOXA1

Additional HNF-3 alpha/FoxA1 Products

Product Documents for HNF-3 alpha/FoxA1 Antibody (S04-5A7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF-3 alpha/FoxA1 Antibody (S04-5A7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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