HNF1 Antibody (4110)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43652

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (98%), Chicken (95%), Porcine (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4110

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HNF1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HNF1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (85%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HNF1 Antibody (4110)

Western Blot: HNF1 Antibody (4110) [NBP2-43652]

Western Blot: HNF1 Antibody (4110) [NBP2-43652]

Western Blot: HNF1 Antibody (4110) [NBP2-43652] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody (4110) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: HNF1 Antibody (4110) [NBP2-43652]

Immunohistochemistry-Paraffin: HNF1 Antibody (4110) [NBP2-43652]

Immunohistochemistry-Paraffin: HNF1 Antibody (4110) [NBP2-43652] - Sample: Paraffin-embedded human SW480. HNF1 alpha antibody (4110) diluted at 1:250.
Western Blot: HNF1 Antibody (4110) [NBP2-43652]

Western Blot: HNF1 Antibody (4110) [NBP2-43652]

Western Blot: HNF1 Antibody (4110) [NBP2-43652] - Analysis of 30 ug whole cell lysate. A: HepG2 7.5% SDS PAGE diluted at 1:1000.

Applications for HNF1 Antibody (4110)

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HNF1

HNF1 is a transcription factor required for the expression of several liver-specific genes. The protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

HNF1 homeobox A, IDDM20, LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor, MODY3, TCF1hepatic, Transcription factor 1

Gene Symbol

HNF1A

Additional HNF1 Products

Product Documents for HNF1 Antibody (4110)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF1 Antibody (4110)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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