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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HNF1A/2087

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment (around aa 214-339) of human HNF1 protein (exact sequence is proprietary) (Uniprot: P20823)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79911)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for HNF1 Antibody (HNF1A/2087)

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777]

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777]

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Small Intestine stained with HNF1 Antibody (HNF1A/2087).
Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777]

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777]

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).
Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777]

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777]

Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).

Applications for HNF1 Antibody (HNF1A/2087)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HNF1

HNF1 is a transcription factor required for the expression of several liver-specific genes. The protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

HNF1 homeobox A, IDDM20, LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor, MODY3, TCF1hepatic, Transcription factor 1

Gene Symbol

HNF1A

UniProt

Additional HNF1 Products

Product Documents for HNF1 Antibody (HNF1A/2087)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HNF1 Antibody (HNF1A/2087)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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