HNF1 Antibody (HNF1A/2087)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79777
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # HNF1A/2087
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 214-339) of human HNF1 protein (exact sequence is proprietary) (Uniprot: P20823)
Localization
Nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79911)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for HNF1 Antibody (HNF1A/2087)
Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777]
Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Small Intestine stained with HNF1 Antibody (HNF1A/2087).Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777]
Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777]
Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).Applications for HNF1 Antibody (HNF1A/2087)
Application
Recommended Usage
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: HNF1
Alternate Names
HNF1 homeobox A, IDDM20, LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor, MODY3, TCF1hepatic, Transcription factor 1
Gene Symbol
HNF1A
UniProt
Additional HNF1 Products
Product Documents for HNF1 Antibody (HNF1A/2087)
Product Specific Notices for HNF1 Antibody (HNF1A/2087)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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