HNF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38243

Novus Biologicals, part of Bio-Techne
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NBP2-38243-25ul
NBP2-38243
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HNF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VYNWFANRRKEEAFRHKLAMDTYSGPPPGPGPGPALPAHSSPGLPPPALSPSKVHGVRYGQPATSETAEVPSSSGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HNF1 Antibody

Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243]

Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243]

Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining in human small intestine and lymph node tissues . Corresponding HNF1A RNA-seq data are presented for the same tissues.
Western Blot: HNF1 Antibody [NBP2-38243]

Western Blot: HNF1 Antibody [NBP2-38243]

Western Blot: HNF1 Antibody [NBP2-38243] - Analysis in human cell lines Caco-2 and U2OS using anti-HNF1A antibody. Corresponding HNF1A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: HNF1 Antibody [NBP2-38243]

Immunocytochemistry/ Immunofluorescence: HNF1 Antibody [NBP2-38243]

Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP2-38243] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
View 4 more images

Applications for HNF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF1

HNF1 is a transcription factor required for the expression of several liver-specific genes. The protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

HNF1 homeobox A, IDDM20, LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor, MODY3, TCF1hepatic, Transcription factor 1

Gene Symbol

HNF1A

UniProt

P20823 (Human)

Additional HNF1 Products

Product Documents for HNF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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