hnRNP C1 + C2 Antibody (4E8)

Novus Biologicals, part of Bio-Techne | Catalog # H00003183-M02-100ug

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all hnRNP C1 + C2 Antibodies »

Product Specifications

Immunogen

HNRNPC (AAH89438.1, 1 a.a. ~ 250 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASNVTNKTDPRSMNSRVFIGNLNTLVVKKSDVEAIFSKYGKIVGCSVHKGFAFVQYVNERNARAAVAGEDGRMIAGQVLDINLAAEPKVNRGKAGVKRSAAEMYGSSFDLDYDFQRDYYDRMYSYPARVPPPPPIARAIKKELTQIKQKVDSLLENLEKIEKEQSKQAVEMKNDKSEEEQSSSSVKKDETNVKMESEGGADDSAEEGDLLDDDDNEDRGDDQLELIKDDEKEAEEGEDDRDSANGEDDS

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for hnRNP C1 + C2 Antibody (4E8)

Western Blot: hnRNP C1 + C2 Antibody (4E8) [H00003183-M02-100ug]

Western Blot: hnRNP C1 + C2 Antibody (4E8) [H00003183-M02-100ug]

Western Blot: hnRNP C1 + C2 Antibody (4E8) [H00003183-M02-100ug] - Detection against Immunogen (54.2 KDa) .
Western Blot: hnRNP C1 + C2 Antibody (4E8) [H00003183-M02-100ug]

Western Blot: hnRNP C1 + C2 Antibody (4E8) [H00003183-M02-100ug]

Western Blot: hnRNP C1 + C2 Antibody (4E8) [H00003183-M02-100ug] - Analysis of HNRNPC expression in transfected 293T cell line by HNRNPC monoclonal antibody (M02), clone 4E8. Lane 1: HNRNPC transfected lysate (Predicted MW: 32.34 KDa). Lane 2: Non-transfected lysate.
ELISA: hnRNP C1 + C2 Antibody (4E8) [H00003183-M02-100ug]

ELISA: hnRNP C1 + C2 Antibody (4E8) [H00003183-M02-100ug]

ELISA: hnRNP C1 + C2 Antibody (4E8) [H00003183-M02-100ug] - Detection limit for recombinant GST tagged HNRNPC is 0.03 ng/ml as a capture antibody.

Applications for hnRNP C1 + C2 Antibody (4E8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: hnRNP C1 + C2

RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. When this is performed, the hnRNPs are isolated (consisting of protein groups named A to U), and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weight starting with 34 kDa up to 43 kDa. The hnRNP-C proteins have a single RNP motif RNA-binding domain (RBD) of 80 to 100 amino acid long. The hnRNP -C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding.4 Although its physiological action is unknown, mutation of the hnRNPC gene causes a embryonic lethal phenotype.

Alternate Names

C1, C2, heterogeneous nuclear ribonucleoprotein C (C1/C2), heterogeneous nuclear ribonucleoproteins C1/C2, HNRNP, hnRNP C1/C2, hnRNPC, HNRPC, MGC104306, MGC105117, MGC117353, MGC131677, nuclear ribonucleoprotein particle C1 protein, nuclear ribonucleoprotein particle C2 protein, SNRPC

Entrez Gene IDs

3183 (Human)

Gene Symbol

HNRNPC

OMIM

164020 (Human)

UniProt

AAH89438.1 (Human)

Additional hnRNP C1 + C2 Products

Product Documents for hnRNP C1 + C2 Antibody (4E8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for hnRNP C1 + C2 Antibody (4E8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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