hnRNP C1 + C2 Antibody (9G1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26221

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9G1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all hnRNP C1 + C2 Antibodies »

Product Summary for hnRNP C1 + C2 Antibody (9G1)

Immunogen

A synthesized peptide derived from Human hnRNP C1 + C2 [UniProt P07910]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP C1 + C2 Antibody (9G1)

hnRNP C1 + C2 Antibody (9G1)

Immunoprecipitation: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] -

Immunoprecipitation: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Immunoprecipitating hnRNP C1 + C2 in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26221 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26221 (3ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (20ug).
hnRNP C1 + C2 Antibody (9G1)

Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] -

Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Image of hnRNP C1 + C2 Antibody (9G1) diluted at 1:66.35 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
hnRNP C1 + C2 Antibody (9G1)

Western Blot: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] -

Western Blot: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate.
All lanes: hnRNP C1 + C2 Antibody at 0.66ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 34, 33, 26, 28 KDa
Observed band size: 42 KDa
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Applications for hnRNP C1 + C2 Antibody (9G1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: hnRNP C1 + C2

RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. When this is performed, the hnRNPs are isolated (consisting of protein groups named A to U), and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weight starting with 34 kDa up to 43 kDa. The hnRNP-C proteins have a single RNP motif RNA-binding domain (RBD) of 80 to 100 amino acid long. The hnRNP -C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding.4 Although its physiological action is unknown, mutation of the hnRNPC gene causes a embryonic lethal phenotype.

Alternate Names

C1, C2, heterogeneous nuclear ribonucleoprotein C (C1/C2), heterogeneous nuclear ribonucleoproteins C1/C2, HNRNP, hnRNP C1/C2, hnRNPC, HNRPC, MGC104306, MGC105117, MGC117353, MGC131677, nuclear ribonucleoprotein particle C1 protein, nuclear ribonucleoprotein particle C2 protein, SNRPC

Gene Symbol

HNRNPC

Additional hnRNP C1 + C2 Products

Product Documents for hnRNP C1 + C2 Antibody (9G1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for hnRNP C1 + C2 Antibody (9G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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