hnRNP F Antibody

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-57273]

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-57273] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.

Immunocytochemistry/immunofluorescence: hnRNP F Antibody [NBP1-57273] -

Immunocytochemistry/immunofluorescence: hnRNP F Antibody [NBP1-57273] - hnRNP F localizes with SMN and PLS3 in fibroblasts and motor neuron processes. Murine embryonic fibroblasts’ (MEFs) cell cultures were derived from Smn +/-; PLS3V5 tg/tg embryos and stained with V5 for PLS3 transgene; hnRNP-F, and F-actin with phalloidin. a) PLS3V5 localized to the same region as hnRNP F. b) hnRNP F similarly localized with SMN. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32938453/), licensed under a CC-BY license.

Western Blot: hnRNP F Antibody [NBP1-57273] -

Western Blot: hnRNP F Antibody [NBP1-57273] - SMN, PLS3, hnRNP F, & hnRNP H1/2 associate in a large complex. a SMN & PLS3 independently associated with hnRNP H1/2 based on co-immunoprecipitation from HEK293T cells transfected with tagged proteins. b Endogenous PLS3, hnRNP F, & hnRNP H 1/2 associated with SMN in mouse brain extracts, based on immunoprecipitation with antisera against endogenous SMN protein. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. c Endogenous SMN, hnRNP F, & hnRNP H1/2 associated with endogenous PLS3 using the same approach outlined in b. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. IP on endogenous mouse brain extract was done from 3 independent brain extractions & preparations. d Size fractionation studies of whole cell lysates from HEK293T cells showed that actin, SMN, PLS3, & hnRNP F and/or H1/2 proteins co-fractionate in complexes of the indicated size (bracket), although the bulk of hnRNP F/H1/2 protein is in other smaller complexes. HEK293T cells were transfected with PLS3-Flag, & hnRNP F-GFP, endogenous hnRNP H1/2, actin, & SMN were detected using antisera Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32938453), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: hnRNP F Antibody [NBP1-57273] -

Western Blot: hnRNP F Antibody [NBP1-57273] - SMN, PLS3, hnRNP F, & hnRNP H1/2 associate in a large complex. a SMN & PLS3 independently associated with hnRNP H1/2 based on co-immunoprecipitation from HEK293T cells transfected with tagged proteins. b Endogenous PLS3, hnRNP F, & hnRNP H 1/2 associated with SMN in mouse brain extracts, based on immunoprecipitation with antisera against endogenous SMN protein. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. c Endogenous SMN, hnRNP F, & hnRNP H1/2 associated with endogenous PLS3 using the same approach outlined in b. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. IP on endogenous mouse brain extract was done from 3 independent brain extractions & preparations. d Size fractionation studies of whole cell lysates from HEK293T cells showed that actin, SMN, PLS3, & hnRNP F and/or H1/2 proteins co-fractionate in complexes of the indicated size (bracket), although the bulk of hnRNP F/H1/2 protein is in other smaller complexes. HEK293T cells were transfected with PLS3-Flag, & hnRNP F-GFP, endogenous hnRNP H1/2, actin, & SMN were detected using antisera Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32938453), licensed under a CC-BY license. Not internally tested by Novus Biologicals.