hnRNP H Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93590

Novus Biologicals, part of Bio-Techne
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NBP2-93590-0.02ml
NBP2-93590-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all hnRNP H Antibodies »

Product Summary for hnRNP H Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 191-449 of human hnRNP H (NP_005511.1). VRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for hnRNP H Antibody - Azide and BSA Free

Western Blot: hnRNP H AntibodyAzide and BSA Free [NBP2-93590]

Western Blot: hnRNP H AntibodyAzide and BSA Free [NBP2-93590]

Western Blot: hnRNP H Antibody [NBP2-93590] - Western blot analysis of extracts of various cell lines, using hnRNP H antibody (NBP2-93590) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody - Azide and BSA Free [NBP2-93590]

Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody - Azide and BSA Free [NBP2-93590]

Immunocytochemistry/Immunofluorescence: hnRNP H Antibody [NBP2-93590] - Immunofluorescence analysis of 293T cells using hnRNP H antibody (NBP2-93590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: hnRNP H Antibody - Azide and BSA Free [NBP2-93590]

Immunohistochemistry-Paraffin: hnRNP H Antibody - Azide and BSA Free [NBP2-93590]

Immunohistochemistry-Paraffin: hnRNP H Antibody [NBP2-93590] - Immunohistochemistry of paraffin-embedded rat ovary using hnRNP H Rabbit pAb (NBP2-93590) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for hnRNP H Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: hnRNP H

The heterogeneous nuclear Ribonucleoprotein (hnRNP) H protein family is involved in pre-mRNA processing. It is essential for enhancer activity. The sequence GGGA is recognized by all hnRNP H family proteins. Mutant exon 6D sequences function as a splicing enhancer when inserted into an enhancer-dependent splicing construct. hnRNP H family proteins bind to the enhancer and their binding is dependent on the sequence GGGA, located just downstream of the point of mutation.

Alternate Names

DKFZp686A15170, heterogeneous nuclear ribonucleoprotein H, heterogeneous nuclear ribonucleoprotein H1 (H), hnRNP H, HNRPH, HNRPH1hnRNPH

Gene Symbol

HNRNPH1

Additional hnRNP H Products

Product Documents for hnRNP H Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for hnRNP H Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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