hnRNP H2 Antibody

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816]

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816] - Staining of human kidney shows nuclear positivity.

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816]

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816] - Staining of human testis shows nuclear positivity.

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816]

Immunohistochemistry-Paraffin: hnRNP H2 Antibody [NBP1-89816] - Staining of human cerebellum shows nuclear positivity in cells in granular layer.

Immunocytochemistry/immunofluorescence: hnRNP H2 Antibody [NBP1-89816] -

Immunocytochemistry/immunofluorescence: hnRNP H2 Antibody [NBP1-89816] - hnRNP H2 localizes with SMN and PLS3 in fibroblasts and motor neuron processes. Murine embryonic fibroblasts’ (MEFs) cell cultures were derived from Smn +/-; PLS3V5 tg/tg embryos and stained with V5 for PLS3 transgene; hnRNP H2 and F-actin with phalloidin. c) hnRNP H2 localized to the same region with tagged PLS3. d) hnRNP H2 localized to the same region with SMN. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32938453/), licensed under a CC-BY license.

Western Blot: hnRNP H2 Antibody [NBP1-89816] -

Western Blot: hnRNP H2 Antibody [NBP1-89816] -Analysis in human cell line MCF-7.

Western Blot: hnRNP H2 Antibody [NBP1-89816] -

SMN, PLS3, hnRNP F, & hnRNP H1/2 associate in a large complex. a SMN & PLS3 independently associated with hnRNP H1/2 based on co-immunoprecipitation from HEK293T cells transfected with tagged proteins. b Endogenous PLS3, hnRNP F, & hnRNP H 1/2 associated with SMN in mouse brain extracts, based on immunoprecipitation with antisera against endogenous SMN protein. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. c Endogenous SMN, hnRNP F, & hnRNP H1/2 associated with endogenous PLS3 using the same approach outlined in b. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. IP on endogenous mouse brain extract was done from 3 independent brain extractions & preparations. d Size fractionation studies of whole cell lysates from HEK293T cells showed that actin, SMN, PLS3, & hnRNP F and/or H1/2 proteins co-fractionate in complexes of the indicated size (bracket), although the bulk of hnRNP F/H1/2 protein is in other smaller complexes. HEK293T cells were transfected with PLS3-Flag, & hnRNP F-GFP, endogenous hnRNP H1/2, actin, & SMN were detected using antisera Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32938453), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: hnRNP H2 Antibody [NBP1-89816] -

SMN, PLS3, hnRNP F, & hnRNP H1/2 associate in a large complex. a SMN & PLS3 independently associated with hnRNP H1/2 based on co-immunoprecipitation from HEK293T cells transfected with tagged proteins. b Endogenous PLS3, hnRNP F, & hnRNP H 1/2 associated with SMN in mouse brain extracts, based on immunoprecipitation with antisera against endogenous SMN protein. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. c Endogenous SMN, hnRNP F, & hnRNP H1/2 associated with endogenous PLS3 using the same approach outlined in b. Arrowheads indicate the band of interest in blots with multiple bands. Heavy & light chain bands are labeled when present. IP on endogenous mouse brain extract was done from 3 independent brain extractions & preparations. d Size fractionation studies of whole cell lysates from HEK293T cells showed that actin, SMN, PLS3, & hnRNP F and/or H1/2 proteins co-fractionate in complexes of the indicated size (bracket), although the bulk of hnRNP F/H1/2 protein is in other smaller complexes. HEK293T cells were transfected with PLS3-Flag, & hnRNP F-GFP, endogenous hnRNP H1/2, actin, & SMN were detected using antisera Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32938453), licensed under a CC-BY license. Not internally tested by Novus Biologicals.