hnRNP L Antibody (4D11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80778

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Amphibian, Bovine, Chicken, Primate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D11

Format

Azide and BSA Free

Concentration

1 mg/ml

View all hnRNP L Antibodies »

Product Summary for hnRNP L Antibody (4D11) - Azide and BSA Free

Immunogen

Human hnRNP proteins from HeLa cells purified by affinity chromatography on ssDNA agarose.

Reactivity Notes

Newt.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for hnRNP L Antibody (4D11) - Azide and BSA Free

Western Blot: hnRNP L Antibody (4D11)Azide and BSA Free [NBP2-80778]

Western Blot: hnRNP L Antibody (4D11)Azide and BSA Free [NBP2-80778]

Western Blot: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778] - Whole cell protein from HeLa (lane 1), A431 (lane 2), NIH-3T3 (lane 3) and PC12 (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was probed with Anti-hnRNP L at 1 ug/ml and detected with an HRP conjugated anti-mouse secondary antibody by chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778]

Immunocytochemistry/ Immunofluorescence: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778]

Immunocytochemistry/Immunofluorescence: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778] - hnRNP L antibody was tested in HeLa cells at 5 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI. Image from the standard format of this antibody.
Immunohistochemistry: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778]

Immunohistochemistry: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778]

Immunohistochemistry: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778] - Immunohistochemistry-Frozen: hnRNP L Antibody (4D11) [NB120-6106] Image from the standard format of this antibody.
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Applications for hnRNP L Antibody (4D11) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunohistochemistry-Frozen

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP L

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (HNRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with HNRNP complexes and along with other HNRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of HNRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions.Strucutrally, hnRPL contains 2 segments of approximately 80 amino acids each which are weakly related to each other and to the ribonucleoprotein consensus sequence-type RNA-binding domains of other HNRNP and snRNP proteins.

Alternate Names

FLJ35509, heterogeneous nuclear ribonucleoprotein L, hnRNP L, hnRNP-L, HNRPL

Gene Symbol

HNRNPL

Additional hnRNP L Products

Product Documents for hnRNP L Antibody (4D11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for hnRNP L Antibody (4D11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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