Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4C9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all hnRNP M Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-261 of human HNRNPM (NP_112480) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for hnRNP M Antibody (OTI4C9) - Azide and BSA Free

Western Blot: hnRNP M Antibody (OTI4C9)Azide and BSA Free [NBP2-71200]

Western Blot: hnRNP M Antibody (OTI4C9)Azide and BSA Free [NBP2-71200]

Western Blot: hnRNP M Antibody (OTI4C9) - Azide and BSA Free [NBP2-71200] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY hnRNP M.
Immunohistochemistry: hnRNP M Antibody (OTI4C9) - Azide and BSA Free [NBP2-71200]

Immunohistochemistry: hnRNP M Antibody (OTI4C9) - Azide and BSA Free [NBP2-71200]

Immunohistochemistry: hnRNP M Antibody (OTI4C9) - Azide and BSA Free [NBP2-71200] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: hnRNP M Antibody (OTI4C9)Azide and BSA Free [NBP2-71200]

Western Blot: hnRNP M Antibody (OTI4C9)Azide and BSA Free [NBP2-71200]

Western Blot: hnRNP M Antibody (OTI4C9) - Azide and BSA Free [NBP2-71200] - Analysis of A549 cell lysate (35ug).
View 24 more images

Applications for hnRNP M Antibody (OTI4C9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: hnRNP M

Proteins that directly bind to nascent RNA polymerase II transcripts--the heterogenous nuclear ribonucleoproteins (hnRNPs)--play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64kDa subunit of cleavage stimulation factor, which is involved in end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

Alternate Names

DKFZp547H118, heterogeneous nuclear ribonucleoprotein M, hnRNP M, HNRNPM4, HNRPM4heterogenous nuclear ribonucleoprotein M, HNRPMheterogenous nuclear ribonucleoprotein M4, HTGR1, M4 protein, N-acetylglucosamine receptor 1, NAGR1hnRNA-binding protein M4

Gene Symbol

HNRNPM

Additional hnRNP M Products

Product Documents for hnRNP M Antibody (OTI4C9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for hnRNP M Antibody (OTI4C9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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