hnRNP M1-M4 Antibody (1D8)

Novus Biologicals, part of Bio-Techne | Catalog # NB200-314

Novus Biologicals, part of Bio-Techne
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NB200-314
NB200-314SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Rabbit

Cited:

Human, Mouse, Rat, Bovine, Rabbit

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D8

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all hnRNP M1-M4 Antibodies »

Product Specifications

Immunogen

M19 fusion protein containing full-length human protein. [UniProt# P52272]

Reactivity Notes

A weak reactivity to mouse has been observed. Mouse reactivity reported in scientific literature (PMID:32931733)

Specificity

NB200-314 is specific for all four M proteins of hnRNP (M1, M2, M3 and M4).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for hnRNP M1-M4 Antibody (1D8)

Western Blot: hnRNP M1-M4 Antibody (1D8) [NB200-314]

Western Blot: hnRNP M1-M4 Antibody (1D8) [NB200-314]

Western Blot: hnRNP M1-M4 Antibody (1D8) [NB200-314] - Analysis of hnRNP M1-M4 in HeLa cell lysates using NB200-314.
Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody (1D8) [NB200-314]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody (1D8) [NB200-314]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody (1D8) [NB200-314] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using hnRNP M1-M4 (1D8) antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes with DAB was used for signal development followed by counterstaining with hematoxylin. Nuclear riboprotein staining was observed.

Applications for hnRNP M1-M4 Antibody (1D8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:500

Reviewed Applications

Read 1 review rated 1 using NB200-314 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: hnRNP M1-M4

RNA-binding proteins of mammalian hnRNP (heterogeneous nuclear ribonucleo protein) family represents a group of over 20 distinct evolutionary conserved proteins which share a capability to bind pre-mRNA/mRNA, and also binds to specific RNA motifs with high-affinity. They exhibit huge functional diversity and implicates in almost every step in mRNA's life cycle (from transcription and processing to transport and translation). HnRNP M is an abundant protein containing three RNA recognition motifs (RRM), a glycine/methionine-rich region and a methionine/arginine-rich repeat motif. It has four protein variants, hnRNP M1-4, ranging in molecular size from 68 to 72 kD which corresponds to spliced isoforms and/or posttranslationally modified forms. hnRNP M is a nucleolar protein and has been Identified in the spliceosome C complex. It acts as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro, and is involved in splicing. hnRNP M acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and TNF-alpha cytokines.

Alternate Names

DKFZp547H118, heterogeneous nuclear ribonucleoprotein M, hnRNP M, HNRNPM4, HNRPM4heterogenous nuclear ribonucleoprotein M, HNRPMheterogenous nuclear ribonucleoprotein M4, HTGR1, M4 protein, N-acetylglucosamine receptor 1, NAGR1hnRNA-binding protein M4

Entrez Gene IDs

4670 (Human); 76936 (Mouse); 116655 (Rat)

Gene Symbol

HNRNPM

UniProt

P52272 (Human)

Additional hnRNP M1-M4 Products

Product Documents for hnRNP M1-M4 Antibody (1D8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for hnRNP M1-M4 Antibody (1D8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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