HOOK3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05070

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HOOK3 Antibodies »

Product Summary for HOOK3 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 489-718 of human HOOK3 (NP_115786.1). IALLQSLLDDANLRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDSVLLKKKLEEHLEKLHEANNELQKKRAIIEDLEPRFNNSSLKIEELQEALRKKEEEMKQMEERYKKYLEKAKSVIRTLDPKQNQGAAPEIQALKNQLQERDRLFHSLEKEYEKTKSQREMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQATSSRRSYPGHVQPATAR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HOOK3 Antibody - Azide and BSA Free

Western Blot: HOOK3 AntibodyAzide and BSA Free [NBP3-05070]

Western Blot: HOOK3 AntibodyAzide and BSA Free [NBP3-05070]

Western Blot: HOOK3 Antibody [NBP3-05070] - Analysis of extracts of various cell lines, using HOOK3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic
Immunocytochemistry/ Immunofluorescence: HOOK3 Antibody - Azide and BSA Free [NBP3-05070]

Immunocytochemistry/ Immunofluorescence: HOOK3 Antibody - Azide and BSA Free [NBP3-05070]

Immunocytochemistry/Immunofluorescence: HOOK3 Antibody [NBP3-05070] - Analysis of L929 cells using HOOK3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: HOOK3 Antibody - Azide and BSA Free [NBP3-05070]

Immunocytochemistry/ Immunofluorescence: HOOK3 Antibody - Azide and BSA Free [NBP3-05070]

Immunocytochemistry/Immunofluorescence: HOOK3 Antibody [NBP3-05070] - Analysis of C6 cells using HOOK3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for HOOK3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:200-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HOOK3

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM]

Alternate Names

FLJ31058, hHK3, HK3h-hook3, hook homolog 3 (Drosophila), protein Hook homolog 3

Gene Symbol

HOOK3

Additional HOOK3 Products

Product Documents for HOOK3 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HOOK3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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