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Hormone-sensitive Lipase/HSL [p Ser853] Antibody (SR2248)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21545

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-21545-100ul
NBP3-21545-50ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2248

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Hormone-sensitive Lipase/HSL [p Ser853] Antibody (SR2248)

Immunogen

A synthesized peptide derived from human phospho-Hormone-sensitive Lipase/HSL [Ser853] (Uniprot #: Q05469)

Modification

p Ser 853

Specificity

Detects endogenous levels of phospho-Hormone-sensitive Lipase/HSL [Ser853]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hormone-sensitive Lipase/HSL [p Ser853] Antibody (SR2248)

Western Blot: Hormone-sensitive Lipase/HSL [p Ser 853] Antibody (SR2248) [NBP3-21545] -

Western Blot: Hormone-sensitive Lipase/HSL [p Ser 853] Antibody (SR2248) [NBP3-21545] - Western blot analysis using NBP3-21545 in (1) Mouse muscle lysate; (2) Mouse muscle lysate treated with AP.

Applications for Hormone-sensitive Lipase/HSL [p Ser853] Antibody (SR2248)

Application
Recommended Usage

Western Blot

1:5000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hormone-sensitive Lipase/HSL

Hormone-sensitive lipase (HSL) is an intracellular neutral lipase found in a variety of tissues, but primarily in adipose and steroidogenic tissues. It hydrolyzes triglycerides into free fatty acids and cholesteryl esters into free cholesterol for steroid hormone synthesis.

HSL is the major enzyme responsible for the mobilization of free fatty acids from adopise tissue, and HSL antibodies are useful tools for lipid and metabolism research.

Alternate Names

Hormonesensitive Lipase, HSL, LHS, LIPE

Gene Symbol

LIPE

Additional Hormone-sensitive Lipase/HSL Products

Product Documents for Hormone-sensitive Lipase/HSL [p Ser853] Antibody (SR2248)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hormone-sensitive Lipase/HSL [p Ser853] Antibody (SR2248)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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