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Host Cell Factor 1/HCFC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16786

Novus Biologicals, part of Bio-Techne
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NBP2-16786

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HCF1. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

209 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Host Cell Factor 1/HCFC1 Antibody

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786]

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786]

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786] - A549 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786]

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786]

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:500.
Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786]

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786]

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for Host Cell Factor 1/HCFC1 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Host Cell Factor 1/HCFC1

This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]

Alternate Names

C1 Factor, CFF, HCF1, HFC1

Gene Symbol

HCFC1

UniProt

Additional Host Cell Factor 1/HCFC1 Products

Product Documents for Host Cell Factor 1/HCFC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Host Cell Factor 1/HCFC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.