HOXB2 Antibody (PCRP-HOXB2-1F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13937

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-HOXB2-1F2

Concentration

0.2 mg/ml

View all HOXB2 Antibodies »

Product Summary for HOXB2 Antibody (PCRP-HOXB2-1F2)

Immunogen

Recombinant fragment (aa114-220) of human HOXB2 (Uniprot: P14652)

Localization

Nucleus, Nucleoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

37.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14063)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HOXB2 Antibody (PCRP-HOXB2-1F2)

Immunocytochemistry/ Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]

Immunocytochemistry/ Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]

Immunocytochemistry/Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HOXB2 Antibody (PCRP-HOXB2-1F2). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]

Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]

Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]

Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937]

Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Flow cytometric analysis of PFA-fixed HeLa cells. HOXB2 antibody (PCRP-HOXB2-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
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Applications for HOXB2 Antibody (PCRP-HOXB2-1F2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HOXB2

The HOXB2 gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcriptio

Alternate Names

homeo box 2H, homeo box B2, homeobox B2, Homeobox protein Hox-2.8, Homeobox protein Hox-2H, homeobox protein Hox-B2, HOX2, HOX2HHox-2.8, K8, K8 home protein

Gene Symbol

HOXB2

UniProt

P14652 (Human)

Additional HOXB2 Products

Product Documents for HOXB2 Antibody (PCRP-HOXB2-1F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HOXB2 Antibody (PCRP-HOXB2-1F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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