HOXD10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28476

Novus Biologicals, part of Bio-Techne
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NBP1-28476
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Equine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all HOXD10 Antibodies »

Product Specifications

Immunogen

Peptide with sequence PNRSCRIEQPVTQQ corresponding to internal region according to NP_002139.2.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HOXD10 Antibody

Immunohistochemistry: HOXD10 Antibody [NBP1-28476]

Immunohistochemistry: HOXD10 Antibody [NBP1-28476]

Immunohistochemistry: HOXD10 Antibody [NBP1-28476] - (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: HOXD10 Antibody [NBP1-28476]

Flow Cytometry: HOXD10 Antibody [NBP1-28476]

Flow Cytometry: HOXD10 Antibody [NBP1-28476] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for HOXD10 Antibody

Application
Recommended Usage

Flow Cytometry

10ug/ml

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Approx. 37 kDa band observed in human kidney lysates (calculated MW of 38.4 kDa band according to NP_002139.2). Additional bands of unknown identity were also consistently observed at 90+26 kDa. These bands were successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows nuclear staining in some epithelial cells of PCT.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HOXD10

This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox D genes located on chromosome 2. The encoded nuclear protein functions as a sequence-specific transcription factor that is expressed in the developing limb buds and is involved in differentiation and limb development. Mutations in this gene have been associated with Wilm's tumor and congenital vertical talus (also known as rocker-bottom foot deformity or congenital convex pes valgus) and/or a foot deformity resembling that seen in Charcot-Marie-Tooth disease.

Long Name

Homeobox D10

Alternate Names

HOX4, HOX4D, HOX4E

Entrez Gene IDs

3236 (Human); 15430 (Mouse); 303991 (Rat)

Gene Symbol

HOXD10

UniProt

NP_002139.2 (Human)

Additional HOXD10 Products

Product Documents for HOXD10 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HOXD10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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