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HOXD13 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52149

Novus Biologicals, part of Bio-Techne
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NBP1-52149

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-KSSFPGDVALNQPD corresponding to internal region according to NP_000514.2.

Epitope

C-KSSFPGDVALNQPD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HOXD13 Antibody

Immunocytochemistry/ Immunofluorescence: HOXD13 Antibody [NBP1-52149]

Immunocytochemistry/ Immunofluorescence: HOXD13 Antibody [NBP1-52149]

Immunocytochemistry/Immunofluorescence: HOXD13 Antibody [NBP1-52149] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP1-52149]

Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP1-52149]

Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP1-52149] - Staining of Human Prostate. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/ Immunofluorescence: HOXD13 Antibody [NBP1-52149]

Immunocytochemistry/ Immunofluorescence: HOXD13 Antibody [NBP1-52149]

Immunocytochemistry/Immunofluorescence: HOXD13 Antibody [NBP1-52149] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). EB09833 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue).

Applications for HOXD13 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

1 - 2 ug/mL

Immunohistochemistry-Paraffin

1 - 2 ug/mL

Peptide ELISA

Detection limit 1:8000
Application Notes
This HOXD13 Antibody is validated for microarray from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-52149 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HOXD13

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. Mutations in this particular gene cause synpolydactyly. [provided by RefSeq]

Alternate Names

BDSD, homeo box 4I, homeo box D13, homeobox D13, Homeobox protein Hox-4I, homeobox protein Hox-D13, HOX4IBDE, SPD

Entrez Gene IDs

3239 (Human); 15433 (Mouse); 288154 (Rat)

Gene Symbol

HOXD13

Additional HOXD13 Products

Product Documents for HOXD13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HOXD13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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