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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Specifications

Immunogen

Recognizes region between residues Between Between 528 and 578.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HPCL Antibody

HPCL Antibody

Western Blot-HPCL Antibody-NBP3-18417-

Western Blot-HPCL Antibody-NBP3-18417-Detection of human and mouse HACL1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HACL1 antibody used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
HPCL Antibody

Immunoprecipitation-HPCL Antibody-NBP3-18417-

Immunoprecipitation-HPCL Antibody-NBP3-18417-Detection of human HACL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HACL1 antibody used for IP at 2 ug per reaction. HACL1 was also immunoprecipitated by rabbit anti-HACL1 antibody. For blotting immunoprecipitated HACL1, was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for HPCL Antibody

Application
Recommended Usage

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HPCL

HPCL catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde

Alternate Names

1600020H07Rik, 2,2-HPCL, 2-hydroxyacyl-CoA lyase 1, 2-hydroxyphytanoyl-CoA lyase2-hydroxyphytanol-CoA lyase, EC 4.1, HPCL, PHYH2FLJ55041, Phytanoyl-CoA 2-hydroxylase 2, phytanoyl-CoA hydroxylase 2

Gene Symbol

HACL1

Additional HPCL Products

Product Documents for HPCL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HPCL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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