Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1E11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HR6B/UBE2B Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2B (NP_003328) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HR6B/UBE2B Antibody (OTI1E11) - Azide and BSA Free

Western Blot: HR6B/UBE2B Antibody (OTI1E11)Azide and BSA Free [NBP2-71337]

Western Blot: HR6B/UBE2B Antibody (OTI1E11)Azide and BSA Free [NBP2-71337]

Western Blot: HR6B/UBE2B Antibody (OTI1E11) - Azide and BSA Free [NBP2-71337] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2B.

Applications for HR6B/UBE2B Antibody (OTI1E11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HR6B/UBE2B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Long Name

Ubiquitin-conjugating Enzyme E2B

Alternate Names

E2-17k, UBE2B

Gene Symbol

UBE2B

Additional HR6B/UBE2B Products

Product Documents for HR6B/UBE2B Antibody (OTI1E11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HR6B/UBE2B Antibody (OTI1E11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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