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HR6B/UBE2B Antibody (PCRP-UBE2B-1C7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07169

Novus Biologicals, part of Bio-Techne
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NBP3-07169-100ug
NBP3-07169-20ug

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-UBE2B-1C7

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length human HR6B/UBE2B protein (Uniprot: P63146)

Reactivity Notes

Predicted to work in Mouse, Rat, Xenopus and Zebrafish.

Localization

Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08912)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HR6B/UBE2B Antibody (PCRP-UBE2B-1C7)

Immunocytochemistry/ Immunofluorescence: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169]

Immunocytochemistry/ Immunofluorescence: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169]

Immunocytochemistry/Immunofluorescence: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169] - Immunofluorescent Analysis of PFA-fixed HeLa cells. HR6B/UBE2B Mouse Monoclonal Antibody (PCRP-HR6B/UBE2B-1C7) followed by IgG-CF488 (green), counterstained with phalloidin.
Flow Cytometry: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169]

Flow Cytometry: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169]

Flow Cytometry: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169] - Flow Cytometric Analysis of PFA-fixed HeLa cells.HR6B/UBE2B Mouse Monoclonal Antibody (PCRP-HR6B/UBE2B-1C7) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169]

Protein Array: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169]

Protein Array: HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [NBP3-07169] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HR6B/UBE2B Mouse Monoclonal Antibody (PCRP-HR6B/UBE2B-1C7).

Applications for HR6B/UBE2B Antibody (PCRP-UBE2B-1C7)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HR6B/UBE2B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Long Name

Ubiquitin-conjugating Enzyme E2B

Alternate Names

E2-17k, UBE2B

Gene Symbol

UBE2B

UniProt

Additional HR6B/UBE2B Products

Product Documents for HR6B/UBE2B Antibody (PCRP-UBE2B-1C7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HR6B/UBE2B Antibody (PCRP-UBE2B-1C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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