HR6B/UBE2B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31293
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Porcine (100%), Rabbit (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (98%), Zebrafish (95%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human HR6B/UBE2B. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (98%).
Localization
Cell membrane , Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HR6B/UBE2B Antibody
![Western Blot: HR6B/UBE2B Antibody [NBP1-31293]](https://resources.bio-techne.com/images/products/HR6B-UBE2B-Antibody-Western-Blot-NBP1-31293-img0014.jpg "Western Blot: HR6B/UBE2B Antibody [NBP1-31293]")
Western Blot: HR6B/UBE2B Antibody [NBP1-31293]
Western Blot: HR6B/UBE2B Antibody [NBP1-31293] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: HR6B/UBE2B Antibody [NBP1-31293]](https://resources.bio-techne.com/images/products/HR6B-UBE2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31293-img0005.jpg "Immunocytochemistry/ Immunofluorescence: HR6B/UBE2B Antibody [NBP1-31293]")
Immunocytochemistry/ Immunofluorescence: HR6B/UBE2B Antibody [NBP1-31293]
Immunocytochemistry/Immunofluorescence: HR6B/UBE2B Antibody [NBP1-31293] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.![Western Blot: HR6B/UBE2B Antibody [NBP1-31293]](https://resources.bio-techne.com/images/products/HR6B-UBE2B-Antibody-Western-Blot-NBP1-31293-img0012.jpg "Western Blot: HR6B/UBE2B Antibody [NBP1-31293]")
Western Blot: HR6B/UBE2B Antibody [NBP1-31293]
Western Blot: HR6B/UBE2B Antibody [NBP1-31293] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells. Used at a dilution of 1:5000.Applications for HR6B/UBE2B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HR6B/UBE2B
Long Name
Ubiquitin-conjugating Enzyme E2B
Alternate Names
E2-17k, UBE2B
Gene Symbol
UBE2B
Additional HR6B/UBE2B Products
Product Documents for HR6B/UBE2B Antibody
Product Specific Notices for HR6B/UBE2B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.