HRAS Antibody (Y13-238) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20119
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rat IgG2a Lambda Clone # Y13-238
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.
Specificity
The antibody reacts with v-HRAS (within amino acids 120-138) and rodent c-HRAS p21's but not v-KRAS or rodent c-KRAS p21's. This antibody also recognizes human c-HRAS and c-KRAS p21's, but not human c-NRAS.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Lambda
Applications for HRAS Antibody (Y13-238) - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: HRAS
Alternate Names
C-BAS/HAS;C-HA-RAS1;C-H-RAS;CTLO;GTPase HRas;HAMSV;HRAS1;H-RASIDX;p21ras;RASH1
Gene Symbol
HRAS
Additional HRAS Products
Product Documents for HRAS Antibody (Y13-238) - Azide and BSA Free
Product Specific Notices for HRAS Antibody (Y13-238) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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