HSD17B2 Antibody (OTI4F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01952

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Simple Western, Western Blot

Cited:

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4F10

Concentration

0.45 mg/ml

View all HSD17B2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSD17B2(NP_002144) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSD17B2 Antibody (OTI4F10)

Western Blot: HSD17B2 Antibody (4F10) [NBP2-01952] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B2.
Flow Cytometry: HSD17B2 Antibody (OTI4F10) [NBP2-01952]

Flow Cytometry: HSD17B2 Antibody (OTI4F10) [NBP2-01952]

Flow Cytometry: HSD17B2 Antibody (4F10) [NBP2-01952] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HSD17B2 antibody, and then analyzed by flow cytometry.
Flow Cytometry: HSD17B2 Antibody (OTI4F10) [NBP2-01952]

Flow Cytometry: HSD17B2 Antibody (OTI4F10) [NBP2-01952]

Flow Cytometry: HSD17B2 Antibody (4F10) [NBP2-01952] - Analysis of Jurkat cells, using anti-HSD17B2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for HSD17B2 Antibody (OTI4F10)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.45 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSD17B2

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol andestrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH

Alternate Names

17-beta-hydroxysteroid dehydrogenase type 2, 20 alpha-hydroxysteroid dehydrogenase, E2DH, EC 1.1.1.62,17-beta-HSD 2, EC 1.1.1.63, EDH17B220-alpha-HSD, estradiol 17-beta-dehydrogenase 2, HSD17, hydroxysteroid (17-beta) dehydrogenase 2, Microsomal 17-beta-hydroxysteroid dehydrogenase, SDR9C2, short chain dehydrogenase/reductase family 9C, member 2, Testosterone 17-beta-dehydrogenase

Entrez Gene IDs

3294 (Human)

Gene Symbol

HSD17B2

Additional HSD17B2 Products

Product Documents for HSD17B2 Antibody (OTI4F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSD17B2 Antibody (OTI4F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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