HSD3B1 Antibody (3C11-D4)

Novus Biologicals, part of Bio-Techne | Catalog # H00003283-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C11-D4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSD3B1 Antibodies »

Product Specifications

Immunogen

HSD3B1 (AAH31999.1, 1 a.a. ~ 373 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKENLKSKTQ

Specificity

HSD3B1 - hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

Marker

Trophoblast-associated Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HSD3B1 Antibody (3C11-D4)

Western Blot: HSD3B1 Antibody (3C11-D4) [H00003283-M01]

Western Blot: HSD3B1 Antibody (3C11-D4) [H00003283-M01]

Western Blot: HSD3B1 Antibody (3C11-D4) [H00003283-M01] - Analysis of HSD3B1 expression in transfected 293T cell line by HSD3B1 monoclonal antibody (M01), clone 3C11-D4.Lane 1: HSD3B1 transfected lysate (Predicted MW: 42.3 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: HSD3B1 Antibody (3C11-D4) [H00003283-M01]

Immunohistochemistry-Paraffin: HSD3B1 Antibody (3C11-D4) [H00003283-M01]

Immunohistochemistry-Paraffin: HSD3B1 Antibody (3C11-D4) [H00003283-M01] - Immunohistochemical staining of various tissues with hematoxylin and HSD3B1 antibody under high magnification.Antibody concentration 3 ug/ml. ( a ) Stomach ( b ) Esophagus ( c ) Endometrium ( d ) Uterine cervix ( e ) Placenta ( f ) Ovary, clear cell carcinoma ( g ) Hepatocellular carcinoma ( h ) Breast cancer ( i ) Colon adenocarcinoma ( j and k ) Cervical carcinoma ( l, m, and n ) Choriocarcinoma ( o ) Epithelioid trophoblastic tumor.
Western Blot: HSD3B1 Antibody (3C11-D4) [H00003283-M01]

Western Blot: HSD3B1 Antibody (3C11-D4) [H00003283-M01]

Western Blot: HSD3B1 Antibody (3C11-D4) [H00003283-M01] - HSD3B1 monoclonal antibody (M01), clone 3C11-D4. Analysis of HSD3B1 expression in human placenta.
View 1 more images

Applications for HSD3B1 Antibody (3C11-D4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSD3B1

3beta-hydroxysteroid dehydrogenase/delta(5)-delta(4)isomerase (HSD3B1) is a bifunctional enzyme involved in the oxidative conversion of steroids and plays an important role in the synthesis of all steroid hormones. There are two HSD3B1 proteins--designated type I and type II--that are expressed by different genes and function in different areas of the body. HSD3B1 has also been shown to be a highly specific and sensitive trophoblast-associated marker.

HSD3B1 is required for the production of progesterone by the placenta, so it is predicted that mutations in this gene may cause infertility in women. HSD3B1 antibodies are useful tools for researchers studying hormone regulation and reproductive biology.

Alternate Names

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3BETAHSD, 3-beta-HSD I, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3BH, delta-5-3-ketosteroid isomerase, EC 1.1.1, HSD3B, HSDB3, HSDB3A, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1, I, progesterone reductase, SDR11E1, short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase, steroid Delta-isomerase, Trophoblast antigen FDO161G

Entrez Gene IDs

3283 (Human)

Gene Symbol

HSD3B1

OMIM

109715 (Human)

UniProt

AAH31999 (Human)

Additional HSD3B1 Products

Product Documents for HSD3B1 Antibody (3C11-D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSD3B1 Antibody (3C11-D4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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