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HSF4 Antibody (1A4)

Novus Biologicals, part of Bio-Techne | Catalog # H00003299-M01

Novus Biologicals, part of Bio-Techne
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H00003299-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HSF4 (NP_001529, 121 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVPALRGDDGRWRPEDLGRLLGEVQALRGVQESTEARLRELRQQNEILWREVVTLRQSHGQQHRVIGKLIQCLFGPLQAGPSNAGGKRKLSLMLDEGSSC

Specificity

HSF4 (1A4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HSF4 Antibody (1A4)

Western Blot: HSF4 Antibody (1A4) [H00003299-M01]

Western Blot: HSF4 Antibody (1A4) [H00003299-M01]

Western Blot: HSF4 Antibody (1A4) [H00003299-M01] - F4 monoclonal antibody (M01), clone 1A4 Western Blot analysis of HSF4 expression in A-431 ( Cat # L015V1 ).
Sandwich ELISA: HSF4 Antibody (1A4) [H00003299-M01] - Detection limit for recombinant GST tagged HSF4 is 0.3 ng/ml as a capture antibody.

Applications for HSF4 Antibody (1A4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSF4

Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described.

Long Name

Heat Shock Transcription Factor 4

Alternate Names

AT-HSFB1, ATHSF4, CTM, HSFB1

Entrez Gene IDs

3299 (Human)

Gene Symbol

HSF4

OMIM

116800 (Human)

Additional HSF4 Products

Product Documents for HSF4 Antibody (1A4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSF4 Antibody (1A4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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