HSP10/EPF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16890

Novus Biologicals, part of Bio-Techne
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NBP2-16890
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (98%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSP10/EPF Antibodies »

Product Specifications

Immunogen

Full length human HSP10/EPF Recombinant protein.

Reactivity Notes

Zebrafish (80%), Japanese Medaka (83%), Xenopus laevis (88%),Chicken (87%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP10/EPF Antibody

Western Blot: HSP10/EPF Antibody [NBP2-16890]

Western Blot: HSP10/EPF Antibody [NBP2-16890]

Western Blot: Hsp10 Antibody [NBP2-16890] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HSP10/EPF Antibody [NBP2-16890]

Immunocytochemistry/ Immunofluorescence: HSP10/EPF Antibody [NBP2-16890]

Immunocytochemistry/Immunofluorescence: HSP10/EPF Antibody [NBP2-16890] - SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 15 min. Green: HSP10 protein stained by HSP10 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: HSP10/EPF Antibody [NBP2-16890]

Immunohistochemistry-Paraffin: HSP10/EPF Antibody [NBP2-16890]

Immunohistochemistry-Paraffin: HSP10/EPF Antibody [NBP2-16890] - Lung carcinoma. Cpn10 antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for HSP10/EPF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSP10/EPF

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. [provided by RefSeq]

Long Name

Heat Shock 10 kDa Protein 1

Alternate Names

Chaperonin 10 Homolog, EPF, GroES, HSPE1

Gene Symbol

HSPE1

UniProt

P61604

Additional HSP10/EPF Products

Product Documents for HSP10/EPF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HSP10/EPF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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