HSP10/EPF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34055

Novus Biologicals, part of Bio-Techne
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NBP2-34055
NBP2-34055-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSP10/EPF Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP10/EPF Antibody

Western Blot: HSP10/EPF Antibody [NBP2-34055]

Western Blot: HSP10/EPF Antibody [NBP2-34055]

Western Blot: HSP10/EPF Antibody [NBP2-34055] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: HSP10/EPF Antibody [NBP2-34055]

Immunohistochemistry-Paraffin: HSP10/EPF Antibody [NBP2-34055]

Immunohistochemistry-Paraffin: HSP10/EPF Antibody [NBP2-34055] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.

Applications for HSP10/EPF Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP10/EPF

Hsp10 make up a family of small heat shock proteins with an approximate molecular mass of 10 kDa (Hsp10s). Also known as Cpn10, Hsp10 acts as a co-chaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, Hsp10 is thought to interact with cHsp60, a reported immunopathological antigen, identifying Hsp10 as a potential contributor to an immunological response.

Long Name

Heat Shock 10 kDa Protein 1

Alternate Names

Chaperonin 10 Homolog, EPF, GroES, HSPE1

Gene Symbol

HSPE1

UniProt

P61604

Additional HSP10/EPF Products

Product Documents for HSP10/EPF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP10/EPF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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