Key Product Details

Species Reactivity

Validated:

Yeast

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A7.H6

Concentration

1 mg/ml

View all HSP40/DNAJB1 Antibodies »

Product Summary for HSP40/DNAJB1 Antibody (2A7.H6)

Immunogen

Full length protein HSP40 (YDJ1)

Localization

Cytoplasm , Nucleus

Specificity

Detects approx 40kDa. Yeast specific.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HSP40/DNAJB1 Antibody (2A7.H6)

Western Blot: HSP40/DNAJB1 Antibody (2A7.H6) [NBP1-22416]

Western Blot: HSP40/DNAJB1 Antibody (2A7.H6) [NBP1-22416]

Western Blot: HSP40/DNAJB1 Antibody (2A7.H6) [NBP1-22416] - Western Blot analysis of Yeast recombinant cell lysate showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 2A7.H6 (NBP1-22416). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP1-22416) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Western Blot: HSP40/DNAJB1 Antibody (2A7.H6) [NBP1-22416]

Western Blot: HSP40/DNAJB1 Antibody (2A7.H6) [NBP1-22416]

Western Blot: HSP40/DNAJB1 Antibody (2A7.H6) [NBP1-22416] - 30ug of protein. Antibody dilutuion of 1:1000. This image was submitted via customer Review.

Applications for HSP40/DNAJB1 Antibody (2A7.H6)

Application
Recommended Usage

Western Blot

1:2000
Application Notes
0.5 ug/ml of HSP40, YDJ1 Antibody was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-22416 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP40/DNAJB1

Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).

Long Name

Heat Shock 40 kDa Protein

Alternate Names

DNAJB1, Hdj1, HSPF1

Entrez Gene IDs

3337 (Human)

Gene Symbol

DNAJB1

UniProt

P25491 (Yeast)

Additional HSP40/DNAJB1 Products

Product Documents for HSP40/DNAJB1 Antibody (2A7.H6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP40/DNAJB1 Antibody (2A7.H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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