HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70969

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1F9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HSP40/DNAJB1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

37.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free

Western Blot: HSP40/DNAJB1 Antibody (OTI1F9)Azide and BSA Free [NBP2-70969]

Western Blot: HSP40/DNAJB1 Antibody (OTI1F9)Azide and BSA Free [NBP2-70969]

Western Blot: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969] - Hsp40/DNAJB1 Antibody (1F9) Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp40 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969]

Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969]

Immunocytochemistry/Immunofluorescence: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp40.
Immunohistochemistry: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969]

Immunohistochemistry: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969]

Immunohistochemistry: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969] - Hsp40/DNAJB1 Antibody (1F9) Staining of paraffin-embedded Human prostate tissue using anti-Hsp40 mouse monoclonal antibody.
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Applications for HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP40/DNAJB1

Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Long Name

Heat Shock 40 kDa Protein

Alternate Names

DNAJB1, Hdj1, HSPF1

Gene Symbol

DNAJB1

Additional HSP40/DNAJB1 Products

Product Documents for HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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