HSP40/DNAJB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38988

Novus Biologicals, part of Bio-Techne
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NBP2-38988-25ul
NBP2-38988
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSP40/DNAJB1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP40/DNAJB1 Antibody

Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody [NBP2-38988]

Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody [NBP2-38988]

Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody [NBP2-38988] - Staining in human adrenal gland and duodenum tissues using anti-DNAJB1 antibody. Corresponding DNAJB1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody [NBP2-38988]

Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody [NBP2-38988]

Immunocytochemistry/Immunofluorescence: HSP40/DNAJB1 Antibody [NBP2-38988] - Staining of human cell line A549 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody [NBP2-38988]

Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody [NBP2-38988]

Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody [NBP2-38988] - Staining of human adrenal gland shows high expression.
View 1 more images

Applications for HSP40/DNAJB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP40/DNAJB1

Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Long Name

Heat Shock 40 kDa Protein

Alternate Names

DNAJB1, Hdj1, HSPF1

Gene Symbol

DNAJB1

UniProt

P25685

Additional HSP40/DNAJB1 Products

Product Documents for HSP40/DNAJB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP40/DNAJB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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