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Key Product Details

Species Reactivity

Yeast

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G10.H8

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length protein yeast HSP40 (YDJ1)

Localization

Cytoplasm , Nucleus

Specificity

Detects approx 40kDa. Yeast specific product.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Hsp40/YDJ1 Antibody (1G10.H8)

Western Blot: Hsp40/YDJ1 Antibody (1G10.H8) [NBP2-12879]

Western Blot: Hsp40/YDJ1 Antibody (1G10.H8) [NBP2-12879]

Western Blot: Hsp40/YDJ1 Antibody (1G10.H8) [NBP2-12879] - Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8 (NBP2-12879). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12879) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications for Hsp40/YDJ1 Antibody (1G10.H8)

Application
Recommended Usage

Western Blot

1:2000
Application Notes
0.5 ug/ml of HSP40, YDJ1 Antibody was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hsp40/YDJ1

0.5 ug/mL was sufficient for detection of 50ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Alternate Names

MAS5, YDJ1 Ydj1p

Gene Symbol

YDJ1

Additional Hsp40/YDJ1 Products

Product Documents for Hsp40/YDJ1 Antibody (1G10.H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hsp40/YDJ1 Antibody (1G10.H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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