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HSP65 (mycobacterial) Antibody (3F7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97880

Novus Biologicals, part of Bio-Techne
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NBP1-97880-0.05ml
NBP1-97880-0.1ml
NBP1-97880-0.2ml

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bacteria, Bovine, Drosophila, Guinea Pig, Hamster, Protozoa

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 3F7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant M. bovis HSP65.

Reactivity Notes

Bacteria (E. coli, M. leprae, M. bovis), cryptosporidium.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP65 (mycobacterial) Antibody (3F7)

Western Blot: HSP65 (mycobacterial) Antibody (3F7) [NBP1-97880]

Western Blot: HSP65 (mycobacterial) Antibody (3F7) [NBP1-97880]

Western Blot: HSP65 (mycobacterial) Antibody (3F7) [NBP1-97880] - Analysis of HSP65: Lane 1: MW marker, Lane 2: GroEL Recombinant E. coli Protein Lane 3: HSP60 Recombinant Human Protein, Lane 4: HSP65 Recombinant Mycobacterium bovis Protein, Lane 5: HeLa (HS) Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: 3T3 Cell Lysate.

Applications for HSP65 (mycobacterial) Antibody (3F7)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP65 (mycobacterial)

Hsp65 is a member of the Hsp60 family of heat shock proteins isolated from Mycobacterium bovis BCG. Hsp65 from M. bovis is identical to that of M. tuberculosis, and similar to that of M. leprae, two highly pathogenic strains of mycobacterium. Hsp65 is the immunodominant antigen during mycobacterial infection and vaccination, and has been linked to the development of autoimmune adjuvant arthritis in rats.

Alternate Names

60 kDa chaperonin 2, 65 kDa antigen, Antigen A, Cell wall protein A, GroEL protein 2, groEL-2, groL2, Heat shock protein 65, hsp65, MT0456, MTV037.04, Protein Cpn60-2, Rv0440

Gene Symbol

groEL2

Additional HSP65 (mycobacterial) Products

Product Documents for HSP65 (mycobacterial) Antibody (3F7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSP65 (mycobacterial) Antibody (3F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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