HSP90 alpha Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77682

Novus Biologicals, part of Bio-Techne
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NBP1-77682
NBP1-77682SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.14 mg/ml

View all HSP90 alpha Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human Hsp90A protein (between residues 1-70) [UniProt P07900]

Localization

Cytoplasm. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP90 alpha Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody - BSA Free [NBP1-77682]

Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody - BSA Free [NBP1-77682]

Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77682] - HSP90-1A antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: HSP90 alpha Antibody - BSA Free [NBP1-77682]

Immunohistochemistry: HSP90 alpha Antibody - BSA Free [NBP1-77682]

Immunohistochemistry: HSP90 alpha Antibody [NBP1-77682] - Analysis of Hsp901a in human kidney carcinoma using DAB with hematoxylin counterstain.

Applications for HSP90 alpha Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 28550115)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.14 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP90 alpha

Heat shock protein 90-1a (HSP90-1a) is a myosin chaperone involved in heat shock responses, as well as in myofibrillogenesis. It is known to interact with histone methyltransferases, influencing epigenetic changes in certain pathways. It interacts with TERT to assemble the telomerase holoenzyme complex, which is critical for addition of DNA to telomeric ends. It also is important in thick and thin filament organization, influencing the formation and maintenance of sarcomere characteristics. It is highly expressed in skeletal and cardiac muscles.

Long Name

Heat Shock Protein 90 alpha

Alternate Names

HSP86, HSP89A, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

Entrez Gene IDs

3320 (Human); 15519 (Mouse)

Gene Symbol

HSP90AA1

UniProt

P07900

Additional HSP90 alpha Products

Product Documents for HSP90 alpha Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP90 alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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