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HSP90 beta Antibody (HSP90AB1/4970) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20694

Novus Biologicals, part of Bio-Techne
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NBP3-20694

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Chicken, Rabbit

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # HSP90AB1/4970

Format

BSA Free

Concentration

1 mg/ml

Product Summary for HSP90 beta Antibody (HSP90AB1/4970) - BSA Free

Immunogen

Recombinant full-length human HSP90 beta protein

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Positive Controls: Human spleen, stomach or pancreas tissue. MCF-7 cells.

Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for HSP90 beta Antibody (HSP90AB1/4970) - BSA Free

HSP90 beta Antibody (HSP90AB1/4970) - BSA Free

HSP90 beta Antibody (HSP90AB1/4970)

Formalin-fixed, paraffin-embedded human prostate stained with HSP90 beta antibody (HSP90AB1/4970). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
HSP90 beta Antibody (HSP90AB1/4970) - BSA Free

HSP90 beta Antibody (HSP90AB1/4970)

Formalin-fixed, paraffin-embedded human thyroid stained with HSP90 beta antibody (HSP90AB1/4970). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for HSP90 beta Antibody (HSP90AB1/4970) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HSP90 beta

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Long Name

Heat Shock Protein 90 beta

Alternate Names

D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Gene Symbol

HSP90AB1

Additional HSP90 beta Products

Product Documents for HSP90 beta Antibody (HSP90AB1/4970) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSP90 beta Antibody (HSP90AB1/4970) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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