HSP90 beta Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77561

Novus Biologicals, part of Bio-Techne
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NBP1-77561
NBP1-77561SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.14 mg/ml

View all HSP90 beta Antibodies »

Product Summary for HSP90 beta Antibody - BSA Free

Immunogen

A synthetic peptide made to an N-terminal portion of the human Hsp90B protein (between residues 1-50) [UniProt P08238]

Localization

Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP90 beta Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody - BSA Free [NBP1-77561]

Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody - BSA Free [NBP1-77561]

Immunocytochemistry/Immunofluorescence: Hsp90B Antibody [NBP1-77561] - HSP90-1B antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: HSP90 beta Antibody - BSA Free [NBP1-77561]

Immunohistochemistry: HSP90 beta Antibody - BSA Free [NBP1-77561]

Immunohistochemistry: HSP90 beta Antibody [NBP1-77561] - Hsp90B Antibody [NBP1-77561] - Staining of HSP901b in mouse prostate using DAB with hematoxylin counterstain.

Applications for HSP90 beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200
Application Notes
This HSP90-1B antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.14 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP90 beta

HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1), also known as HSPC2, HSPCB and HSP90B, is a chaperone that plays an integral part in signal transduction, protein folding and degradation, cell cycle regulation, and protein maturation. Specifically, HSP90AB1 is a highly conserved chaperone which interacts with other cochaperones to assist in either the folding or refolding of proteins. HSP90AB1 is a constitutive form of the two major HSP90 proteins and is found primarily in the cytosol along with the inducible form, HSP90AA1. HSP90B is a target molecule for treatment of Helicobacter pylori (H. pylori)-induced gastric injury due to an interaction of HSP90B1 with Rac1 ultimately leading to increased production in reactive oxygen species (ROS) in gastric epithelial cells (PMID: 20451655). HSP90B in conjunction with HSP70 also plays a role in HIF-1alpha stability via ubiquitin and O(2)-independent regulation (PMID: 19802386). HSP90 antibodies are useful tools for studying cancer or other diseases brought on by cellular stress.

Long Name

Heat Shock Protein 90 beta

Alternate Names

D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Entrez Gene IDs

3326 (Human); 15516 (Mouse)

Gene Symbol

HSP90AB1

UniProt

P08238

Additional HSP90 beta Products

Product Documents for HSP90 beta Antibody - BSA Free

Product Datasheets

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Product Specific Notices for HSP90 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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