HSP90 beta Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77563

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all HSP90 beta Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human Hsp90B protein (between residues 650-724) [UniProt P08238]

Reactivity Notes

Immunogen has 86% identity to cow.

Localization

Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP90 beta Antibody - BSA Free

Knockdown Validated: HSP90 beta Antibody - BSA Free [NBP1-77563]

Knockdown Validated: HSP90 beta Antibody - BSA Free [NBP1-77563]

Knockdown Validated: HSP90 beta Antibody [NBP1-77563] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP1-77563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: HSP90 beta AntibodyBSA Free [NBP1-77563]

Western Blot: HSP90 beta AntibodyBSA Free [NBP1-77563]

Western Blot: HSP90 beta Antibody [NBP1-77563] - Analysis of HSP901b in: 1. HeLa, 2. Ntera 2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. PC12 and 8. COS7.
Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody - BSA Free [NBP1-77563]

Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody - BSA Free [NBP1-77563]

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP1-77563] - HSP90-1B antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
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Applications for HSP90 beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Simple Western

1:1000

Western Blot

1 ug/ml
Application Notes
This HSP90-1B antibody is useful for Immunocytochemistry/Immunofluorescence, IHC-paraffin embedded sections, and Western blot where a band is seen ~83 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP90 beta

HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1), also known as HSPC2, HSPCB and HSP90B, is a chaperone that plays an integral part in signal transduction, protein folding and degradation, cell cycle regulation, and protein maturation. Specifically, HSP90AB1 is a highly conserved chaperone which interacts with other cochaperones to assist in either the folding or refolding of proteins. HSP90AB1 is a constitutive form of the two major HSP90 proteins and is found primarily in the cytosol along with the inducible form, HSP90AA1. HSP90B is a target molecule for treatment of Helicobacter pylori (H. pylori)-induced gastric injury due to an interaction of HSP90B1 with Rac1 ultimately leading to increased production in reactive oxygen species (ROS) in gastric epithelial cells (PMID: 20451655). HSP90B in conjunction with HSP70 also plays a role in HIF-1alpha stability via ubiquitin and O(2)-independent regulation (PMID: 19802386). HSP90 antibodies are useful tools for studying cancer or other diseases brought on by cellular stress.

Long Name

Heat Shock Protein 90 beta

Alternate Names

D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Entrez Gene IDs

3326 (Human); 15516 (Mouse); 301252 (Rat); 767874 (Bovine)

Gene Symbol

HSP90AB1

UniProt

P08238

Additional HSP90 beta Products

Product Documents for HSP90 beta Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP90 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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