HSP90 beta Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55509
![Western Blot: HSP90 beta Antibody [NBP2-55509]](https://resources.bio-techne.com/images/products/HSP90AB1-Antibody-Western-Blot-NBP2-55509-img0003.jpg "Western Blot: HSP90 beta Antibody [NBP2-55509]")
Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DEDEVAAEEPNAAVPDEIPPLEGDEDASR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HSP90 beta Antibody
![Knockout Validated: HSP90 beta Antibody [NBP2-55509]](https://resources.bio-techne.com/images/products/HSP90-beta-Antibody-Knockdown-Validated-NBP2-55509-img0004.jpg "Knockout Validated: HSP90 beta Antibody [NBP2-55509]")
Knockout Validated: HSP90 beta Antibody [NBP2-55509]
Knockout Validated: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.![Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody [NBP2-55509]](https://resources.bio-techne.com/images/products/HSP90AB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55509-img0001.jpg "Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody [NBP2-55509]")
Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody [NBP2-55509]
Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP2-55509] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.Applications for HSP90 beta Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HSP90 beta
Long Name
Heat Shock Protein 90 beta
Alternate Names
D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB
Gene Symbol
HSP90AB1
Additional HSP90 beta Products
Product Documents for HSP90 beta Antibody
Product Specific Notices for HSP90 beta Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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