HSPA14 Antibody (4L1Z6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16107
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4L1Z6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HSPA14 (Q0VDF9). MAAIGVHLGCTSACVAVYKDGRAGVVANDAGDRVTPAVVAYSENEEIVGLAAKQSRIRNISNTVMKVKQILGRSSSDPQAQKYIAESKCLVIEKNGKLRY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HSPA14 Antibody (4L1Z6)
Western Blot: HSPA14 Antibody (4L1Z6) [NBP3-16107]
Western Blot: HSPA14 Antibody (4L1Z6) [NBP3-16107] - Western blot analysis of extracts of various cell lines, using HSPA14 Rabbit mAb (NBP3-16107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: HSPA14 Antibody (4L1Z6) [NBP3-16107]
Immunocytochemistry/Immunofluorescence: HSPA14 Antibody (4L1Z6) [NBP3-16107] - Immunofluorescence analysis of NIH-3T3 cells using HSPA14 Rabbit mAb (NBP3-16107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: HSPA14 Antibody (4L1Z6) [NBP3-16107]
Immunohistochemistry-Paraffin: HSPA14 Antibody (4L1Z6) [NBP3-16107] - Immunohistochemistry of paraffin-embedded human colon using HSPA14 Rabbit mAb (NBP3-16107) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for HSPA14 Antibody (4L1Z6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HSPA14
Alternate Names
heat shock 70kDa protein 14, HSP70-4, HSP70L1
Gene Symbol
HSPA14
Additional HSPA14 Products
Product Documents for HSPA14 Antibody (4L1Z6)
Product Specific Notices for HSPA14 Antibody (4L1Z6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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