HspA1L Antibody (7D3-R)

Western Blot: HspA1L Antibody (7D3-R) [NBP3-32447]

Western Blot: HspA1L Antibody (7D3-R) [NBP3-32447] - Western blot analysis of HspA1L on different lysates with Mouse anti-HspA1L antibody (NBP3-32447) at 1/2,000 dilution.

Lane 1: NCI-H1299 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: Mouse testis tissue lysate (40 ug/Lane)
Lane 4: Rat testis tissue lysate (40 ug/Lane)

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: Lane 1-2: 5 minutes; Lane 3-4: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32447) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: HspA1L Antibody (7D3-R) [NBP3-32447]

Immunohistochemistry: HspA1L Antibody (7D3-R) [NBP3-32447] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-HspA1L antibody (NBP3-32447) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32447) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: HspA1L Antibody (7D3-R) [NBP3-32447]

Immunocytochemistry/ Immunofluorescence: HspA1L Antibody (7D3-R) [NBP3-32447] - Immunocytochemistry analysis of HeLa cells labeling HspA1L with Mouse anti-HspA1L antibody (NBP3-32447) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HspA1L antibody (NBP3-32447) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.