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HspA4L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48700

Novus Biologicals, part of Bio-Techne
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NBP2-48700
NBP2-48700-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KCHAEHTPEEEIDHTGAKTKSAVSDKQDRLNQTLKKGKVKSIDLPIQSSLCRQLGQDLLNSY

Reactivity Notes

Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HspA4L Antibody

Immunohistochemistry-Paraffin: HspA4L Antibody [NBP2-48700]

Immunohistochemistry-Paraffin: HspA4L Antibody [NBP2-48700]

Immunohistochemistry-Paraffin: HspA4L Antibody [NBP2-48700] - Staining in human testis and endometrium tissues using anti-HSPA4L antibody. Corresponding HSPA4L RNA-seq data are presented for the same tissues.
Western Blot: HspA4L Antibody [NBP2-48700]

Western Blot: HspA4L Antibody [NBP2-48700]

Western Blot: HspA4L Antibody [NBP2-48700] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: HspA4L Antibody [NBP2-48700]

Immunocytochemistry/ Immunofluorescence: HspA4L Antibody [NBP2-48700]

Immunocytochemistry/Immunofluorescence: HspA4L Antibody [NBP2-48700] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.

Applications for HspA4L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HspA4L

HSPA4L is a novel autocrine pathway of CD1d regulation by Hsp110.

Alternate Names

APG1, APG-1, heat shock 70 kDa protein 4L, heat shock 70 kDa protein 4-like protein, heat shock 70kDa protein 4-like, Heat shock 70-related protein APG-1, heat shock protein (hsp110 family), Osmotic stress protein 94, Osp94

Gene Symbol

HSPA4L

Additional HspA4L Products

Product Documents for HspA4L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HspA4L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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