HSPA8/HSC71/Hsc70 Antibody (0J2Z9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15262

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15262-100ul
NBP3-15262-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0J2Z9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSPA8/HSC71 Antibodies »

Product Summary for HSPA8/HSC71/Hsc70 Antibody (0J2Z9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 547-646 of human HSPA8/HSC71/Hsc70 (P11142). FNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSPA8/HSC71/Hsc70 Antibody (0J2Z9)

Western Blot: HSPA8/HSC71/Hsc70 Antibody (0J2Z9) [NBP3-15262]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (0J2Z9) [NBP3-15262]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (0J2Z9) [NBP3-15262] - Western blot analysis of extracts of various cell lines, using HSPA8/HSC71/Hsc70 Rabbit mAb (NBP3-15262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for HSPA8/HSC71/Hsc70 Antibody (0J2Z9)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSPA8/HSC71

Hsp73 is a normal cell constituent which is synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues (1). Data has shown a molecular mechanism for cytokine-mediated posttranscriptional regulation of Bim mRNA by Hsc70, which binds to AU-rich elements (AREs) in the 3'-untranslated region of specific mRNAs and enhances their stability. The RNA binding potential of Hsc70 is regulated by cochaperones including Bag-4 (also SODD), CHIP, Hip, and Hsp40 (2). Hsp73 protein expression was detected in the cytoplasm of melanoma cells in primary tumors and metastases. In primary melanomas, the proportion of Hsp73 protein expressing cells correlated with tumor thickness which was found to be statistically significant (3).

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Gene Symbol

HSPA8

Additional HSPA8/HSC71 Products

Product Documents for HSPA8/HSC71/Hsc70 Antibody (0J2Z9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSPA8/HSC71/Hsc70 Antibody (0J2Z9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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