HSPA8/HSC71/Hsc70 Antibody (1F2-H5)

Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.

Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880]

Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degrees C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining.

Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880]

Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.